• Exam Code: ASCP-MLT
  • Exam Name: MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
  • Certification Provider: ASCP
  • Corresponding Certification:MLT(ASCP)
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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Topic 1: Blood Banking (Immunohematology)15-20%- Compatibility testing
- Blood group systems and antigens
- Antibody detection and identification
- Blood components and storage
- Transfusion reactions and adverse events
Topic 2: Immunology and Serology5-10%- Infectious disease serology
- Immunologic testing methodologies
- Immune system principles and components
- Autoimmune and immunodeficiency disorders
Topic 3: Laboratory Operations5-10%- Quality control and quality assurance
- Laboratory mathematics and instrumentation
- Pre-analytical, analytical, and post-analytical processes
- Laboratory safety and regulatory compliance
Topic 4: Hematology20-25%- Hemostasis and coagulation testing
- White blood cell morphology and diseases
- Routine and automated hematology procedures
- Red blood cell physiology and disorders
Topic 5: Clinical Chemistry20-25%- Endocrinology and therapeutic drug monitoring
- Carbohydrates, lipids, proteins, and enzymes
- Toxicology and liver/kidney function tests
- Electrolytes, acid-base balance, and blood gases
Topic 6: Microbiology15-20%- Identification of pathogenic microorganisms
- Specimen collection and culture techniques
- Bacteriology and antimicrobial susceptibility
- Mycology, parasitology, and virology
Topic 7: Urinalysis and Other Body Fluids5-10%- Cerebrospinal, serous, and synovial fluids
- Urine formation and composition
- Semen and amniotic fluid testing
- Physical, chemical, and microscopic analysis

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. High-sensitivity C-reactive protein (hs-CRP) is used as an aid in the diagnosis and treatment of cardiovascular disease (CVD). At low levels, it can detect those at risk for cardiac heart disease. At high levels in those with no history of heart disease, it indicates a high risk for acute myocardial infarction (AMI), stroke, or peripheral vascular disease. For patients with acute coronary syndrome (ACS) or stable coronary disease, hs-CRP is used to predict future coronary events.
Ranges of hs-CRP in prediction of risk for CVD are:
<1.0 mg/L Low CVD risk
1.0-3.0 mg/L Average risk for CVD
>3.0 mg/L High risk for future CVD
If results are >10.0 mg/L, the patient should be evaluated for an acute inflammatory condition.
Chemistry
A high-sensitivity C-reactive protein (hs-CRP) test result of 5 mg/L (normal = < 1 mg/L) may indicate which of the following?

A) High risk for future CVD
B) Low risk for cardiovascular disease (CVD)
C) Acute inflammatory condition


2. Each of the drugs/drug classes listed above have been known to cause drug-induced hemolytic anemia, although cephalosporins are the MOST COMMON cause. Cephalosporins can cause drug-induced hemolytic anemia when a patient produces antibodies to the particular cephalosporin drug in the presence of red blood cells. The drugs can alter the membrane appearance of the red blood cells, causing the body to mistake them as foreign. Complement becomes activated due to these antibodies; red cells are then destroyed causing hemolytic anemia. Dark urine, caused by intravascular hemolysis, is one of the most common symptoms associated with this condition.
Which one of the following drugs/drug classes is the MOST COMMON cause of drug-induced hemolytic anemia?

A) Cephalosporins
B) Quinidine
C) Levofloxacin
D) Nonsteroidal anti-inflammatory drugs (NSAIDs)
E) Levodopa


3. The primary mechanism responsible for glomerular filtration is:

A) Concentration of blood components
B) Rate of blood flow through the kidneys
C) Osmotic gradient
D) Hydrostatic differential in glomerular tufts


4. This Gram stain is under-decolorized
This microscopic field is representative of other fields that were observed on a Gram-stained smear. Which of the following describes the quality of the smear?

A) Over-decolorized
B) Under-decolorized
C) Sufficiently decolorized


5. Serum amylase and lipase levels may be slightly elevated in chronic pancreatitis, but not diagnostic enough to predict chronic pancreatitis; wheras high levels are found only during acute pancreatitis episodes. In the later stages of chronic pancreatitis, normal to decreased levels of amylase and lipase are caused by the gradual inability of the pancreas to secrete the enzyme All of the statements below regarding amylase and lipase in pancreatitis are TRUE EXCEPT:

A) Amylase and lipase are as predictive in chronic as in acute pancreatitis.
B) Serum lipase peaks at 24 hours after an episode of acute pancreatitis and remains high for 7-8 days.s.
C) Diagnosis sensitivity is increased by assaying both amylase and lipase.
D) Urinary amylase: creatinine ratio is the most sensitive test for acute pancreatitis.


Solutions:

Question # 1
Answer: A
Question # 2
Answer: A
Question # 3
Answer: D
Question # 4
Answer: B
Question # 5
Answer: A

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