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ASCP ASCP-MLT Exam Syllabus Topics:

SectionObjectives
Clinical Chemistry- Metabolic and analytical testing
  • 1. Endocrine and metabolic panels
    • 2. Enzymes and electrolytes
      Blood Banking / Immunohematology- Blood group systems and antibody identification
      • 1. Crossmatching procedures
        • 2. ABO and Rh systems
          Laboratory Operations- Quality and safety in the clinical lab
          • 1. Laboratory safety and regulations
            • 2. Quality control and assurance
              Microbiology- Clinical microbiological techniques
              • 1. Bacteriology and organism identification
                • 2. Culture and sensitivity testing
                  Hematology- Blood cell analysis and disorders
                  • 1. CBC and differential interpretation
                    • 2. Anemias and leukemias overview
                      Urinalysis and Body Fluids- Fluid analysis and interpretation
                      • 1. Urine chemical and microscopic examination
                        • 2. CSF and other body fluids

                          ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

                          Question 1

                          Name the comprehensive written safety plan detailing the proper use and storage of hazardous chemicals in the workplace.

                          A. SOP
                          B. MSDS
                          C. chemical hygiene plan
                          D. OSHA


                          Question 2

                          Neutrophils, lymphocytes and macrophage/ monocytes can be found in all types of body fluid differentials.
                          Bronchial cells can be found only in bronchial washings and BAL specimens. Mesothelial cells are found only in serous body fluids including pleural fluid, peritoneal fluid, and pericardial fluid.
                          Select the specific cells listed below that can be found in all types of body fluid.

                          A. Lymphocytes
                          B. Mesothelial cells
                          C. Neutrophils
                          D. Bronchial lining cells
                          E. Macrophage/monocytes


                          Question 3

                          The correct answer is Trichophyton rubrum. The tiny microconidia are lined up in a "birds on the fence" arrangement along the hyphal strand transversing the field of view (yellow arrows). Two pencil-shaped, smooth walled macroconidia are also seen in the lower left field of view (red arrows).
                          Trichophyton verrucosum may produce microconidia in small quantities. However, they are irregularly arranged with little tendency to line up along the hyphae. Antler hyphae and string bean macroconidia are characteristic of this species.
                          One of the key characteristics in the identification of Epidermophyton floccosum is the inability of this dermatophyte to produce microconidia. Two to four-celled, club-shaped macroconidia are produced, usually in clusters of two or three.

                          The profusion of tiny microconidia lining up along the hyphae in this photomicrograph is characteristic of which of the following organisms?

                          A. Trichophyton verrucosum
                          B. Trichophyton rubrum
                          C. Epidermophyton floccosum


                          Question 4

                          Clostridium difficile is a gram-positive, anaerobic, spore-forming bacillus that produces a strong toxin. C difficile is a common cause of antibiotic-associated diarrhea and pseudomembranous colitis.
                          Microbiology
                          A recto-sigmoidoscopy revealed pseudomembranes in a patient with severe diarrhea following prolonged treatment with ampicillin. Which of the following organisms is MOST likely to be isolated?

                          A. Klebsiella oxytoca
                          B. Clostridium botulinum
                          C. Clostridium difficile
                          D. Fusobacterium nucleatum
                          E. Bacteroides fragilis


                          Question 5

                          In DNA complementary base pairing, guanine and cytosine pair and adenine and thymine base pair.
                          Remembering the phrase "G-CAT" helps one recollect correct pairing.
                          Which nitrogen base would bind with a guanine nucleotide in forming double-stranded DNA?

                          A. Adenine
                          B. Uracil
                          C. Cytosine
                          D. Thymine


                          Solutions:

                          Question 1
                          Answer: C
                          Question 2
                          Answer: A,C,E
                          Question 3
                          Answer: B
                          Question 4
                          Answer: C
                          Question 5
                          Answer: C

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